Genetic Analysis of Rhododendron Mutants Using Random Amplified Polymorphic Dna (rapd)
نویسندگان
چکیده
The aim of our study was to consider the efficiency of randomly amplified polymorphic DNA (RAPD) analysis to show the differences between Rhododendron mutants and control plants. The mutant plantlets were selected due to their better In vitro shoot and root regeneration relative to control in both varieties. Six mutant plantlets from Alfred and seven mutant plantlets from Paars variety were selected from M1V2 generations. These mutants were selected from plantlets which were irradiated with doses 5 and 10 Gy. They were grown up In vitro conditions. The dendrogram shows one big cluster and the others to be distinguished. Genetic variability induced with gamma ray and RAPD methods were used to detect mutations at the Rhododendron shoot culture. Our results show that Rhododendron mutants were district from controls.
منابع مشابه
Genetic diversity among Elaeagnus angustifolia L. populations based on some morphological traits and Random Amplified Polymorphic DNA Markers
Elaeagnus angustifolia L. is a Eurasian tree that has become naturalized and has various ecological, medicinal and economical uses. In this study, a combination of morphological traits and RAPD markers were used to study the presence or absence of an association between genetic variation and morphological features among five populations of E. angustifolia collected from the East Azarbaijan of I...
متن کاملAnalysis of Induced Mutants of Salinity Resistant Banana (Musa acuminata cv. Dwarf Cavendish) Using Morphological and Molecular Markers
Genetic diversity amongst 21 induced mutant clones tolerant to salinity along with one non-irradiated sensitive clone of banana (Musa acuminata cv. Dwarf Cavendish (AAA)) were studied using morphological and random amplified polymorphic DNA (RAPD) markers. Out of the 30 phenotypic indices screened, 23 were polymorph and two traits, leaf habit and blotches color, were differentiated by non-irrad...
متن کاملStudy of Genetic Diversity among Philodendron Varieties by Randomly Amplified Polymorphic DNA Markers
In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...
متن کاملCharacterization of Pseudomonas aeruginosa in Burn Patients Using PCR- Restriction Frag-ment Length Polymorphism and Random Amplified Polymorphic DNA Analysis
One of the major opportunistic pathogens in patients with burn injuries is Pseudomonas aeruginosa, which causes severe infections in burned patients. The objective of the study was to examine the molecular epidemiology of P. aeruginosa colonization in the burn unit of Shahid Motahari Hospital in Tehran, Iran. Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (...
متن کاملMolecular differentiation of sheep and cattle isolates of Fasciola hepatica using RAPD-PCR
Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected ani...
متن کامل